TBS-T or PBS-T, does it matter which buffer is used for antibody incubations?-标准-资讯-生物在线
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TBS-T or PBS-T, does it matter which buffer is used for antibody incubations?

作者：Agrisera AB 2020-10-21T00:00 (访问量:1896)

 TBS-T or PBS-T, does it matter which buffer is used for antibody incubations? 

Oftentimes TBS-T is a buffer of choice applied in many Western blot protocols for all antibody incubations. 

However, as you can see in this example, changing to PBS-T has also changed a pattern of detected bands. Analysed samples (Arabidopsis thaliana), protein load/well, primary, secondary antibodies and detection reagent were kept the same. 

Antibody incubation buffer has been changed from TBS-T (left pannel) to PBS-T(right panel).
 


 

   Western blot with anti-catalase antibodies


Western blot protocol with all details for this experiment can be found here. 

 

 

 

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