犬细小病毒(CPV)英文说明书定性-分析方法-资讯-生物在线

犬细小病毒(CPV)英文说明书定性

作者:上海逸峰生物科技有限公司 2011-11-08T00:00 (访问量:4133)

     canine parvovirus

FOR RESEARCH USE ONLY

Drug Names

Generic NameCPV ELISA Kit.

Purpose

This kit allows for the determination of CPV concentrations in canine serum, and other biological fluids.

Principle of the assay

The kit assay CPV level in the sampleuse Purified CPV antibody to coat microtiter plate wells, make solid-phase antibody, then add CPV to wells, Combined With CPV, after washing and removing non-combinative antibody and other components ,then Combined CPV antibody which with HRP labeled become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. Compared with the CUTOFF value, according to this to judge CPV exist in the sample or not.

Materials provided with the kit

Materials provided with the kit

48determinations

96 determinations

Storage

User manual

1

1

Closure plate membrane

2

2

Sealed bags

1

1

Microelisa stripplate

1

1

2-8

Negative control

0.5ml×1 bottle

0.5ml×1 bottle

2-8

Positive control

0.5ml×1 bottle

0.5ml×1 bottle

2-8

HRP-Conjugate reagent

3ml×1 bottle

6ml×1 bottle

2-8

Sample diluent

3ml×1 bottle

6ml×1 bottle

2-8

Chromogen Solution A

3ml×1 bottle

6ml×1 bottle

2-8

Chromogen Solution B

3ml×1 bottle

6ml×1 bottle

2-8

Stop Solution

3ml×1 bottle

6ml×1 bottle

2-8

wash  solution

20ml×20 fold

×1bottle

20ml×30 fold

×1bottle

2-8

Specimen requirements

1.       serum- coagulation at room temperature 10-20 minscentrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

2.       plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20 mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

3.       Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The Operation of Hydrothorax and cerebrospinal fluid Reference to it.

4.       cell culture supernatant-detect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,detect the composition of cells, Dilut cell suspension with PBSPH7.2-7.4, Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells and release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

5.       Tissue samples- After cutting samples, check the weight,add PBSPH7.2-7.4, Rapidly frozen with liquid nitrogen, maintain samples at 2-8

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