Epicypher新品 | 核酸提取缓冲液
CUTANA™ 细胞核提取缓冲液是CUT&RUN和CUT&Tag实验中，用于从培养的细胞和组织中提取细胞核的必需试剂。这种缓冲液可与多种真核细胞和组织兼容。以下是使用该缓冲液成功进行细胞核分离的细胞和组织类型：
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mouse NIH3T3 fibroblast cells |
human A549 non-small cell lung cancer (NSCLC) cells |
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human K562 leukemia cells |
human NCI-H1299 non-small cell lung cancer (NSCLS cells) |
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human bone marrow derived macrophages |
human TIG-1 fetal lung cells |
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human monocyte derived macrophages |
human LoVo colorectal cancer cells |
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human MV-4-11 macrophage cells |
human LNCaP prostate carcinoma cells |
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human SUM149 triple negative breast cancer (TNBC) cells |
human renal primary cells |
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human GM24385 B-lymphocyte (aka HG002) cells |
human peripheral blood mononuclear cells (PBMCs) |
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human MCF7 breast cancer cells |
human intestinal tissue |
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human MDA-MB-231 breast cancer cells |
human HEPM embryonic cells |
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human SK-MEL-2 melanoma cells |
只需将试剂盒中的Pre-Nuclei Extraction Buffer和亚精胺与蛋白酶抑制剂混合即可完成该试剂的制备。CUTANA™ 细胞核提取缓冲液针对CUT&RUN和CUT&Tag实验流程进行了优化，从而获得高质量的细胞核，助力您的基因组图谱分析。
试剂盒组成
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组分 |
货号 |
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CUTANA™ Pre-Nuclei Extraction Buffer |
21-1026a |
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1 M Spermidine |
21-1026b |
保存条件
将Pre-Nuclei Extraction Buffer 4ºC储存, 1 M亚精胺-20ºC储存。
自收到之日起可稳定储存6个月。
验证数据
Figure 1: Nuclei Extraction
Nuclei were extracted from 3 different cell types: K562, NIH3T3, and LNCaP using the CUTANA™ Nuclei Extraction Protocol for CUT&RUN and CUT&Tag. Top 3 panels show cells before extraction. Starting cells are viable (bright white and round). Bottom 3 panels show nuclei extracted using the CUTANA™ Nuclei Extraction Buffer as indicated by positive Trypan Blue staining.
订购详情
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货号 |
产品名称 |
规格 |
|
21-1026 |
CUTANA™ Nuclei Extraction Buffer |
100 Reactions |
如需了解更多详细信息或相关产品，请联系EpiCypher中国授权代理商-欣博盛生物
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