肉毒碱棕榈酰基转移酶1A(CPT1A)重组蛋白说明书

Recombinant Carnitine Palmitoyltransferase 1A, Liver (CPT1A) 

[ DESCRIPTION]

Protein Names: Carnitine Palmitoyltransferase 1A,Liver

Synonyms: CPT1A, CPT1

 Species: Human

Size: 100µg

Source: Escherichia coli-derived

Subcellular Location: Mitochondrion outermembrane; Multi-pass membrane protein.

[ PROPERTIES ]

Residues: Arg191~Leu353 with N-terminal His-Tag. 

Grade &Purity: >95%, 23kDa as determined bySDS-PAGE reducing conditions.

Formulation: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose.

Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0 EU per 1μg (determined bythe LAL method).

 Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted Molecular Mass: 21.0kDa

Predicted  isoelectric point: 9.5

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8ºC for one month.

Aliquot and store at -80ºC for 12 months.

 Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37ºC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES  ] 

The target protein is fused with N-terminal His-Tag, its sequence is listed below. 

MGHHHHHHSGSEF-RPLMKEEDFK RMTALAQDFA VGLGPRLQWY LKLKSWWATNYVSDWWEEYI YLRGRGPLMV NSNYYAMDLL YILPTHIQAA RAGNAIHAIL LYRRKLDREEIKPIRLLGST IPLCSAQWER MFNTSRIPGE ETDTIQHMRD SKHIVVYHRG RYFKVWLYHDGRL

本产品仅供科研使用。不能用于人和动物治疗等其它临床诊断使用