灵敏的ELISA试剂盒，能够检测任何物种的培养上清液和血浆中的12(S)-HETE

HETE是由脂氧合酶代谢花生四烯酸产生的副产物。12(S)-HETE是12(S)-氢过氧四烯酸（12(S)-HpETE）还原的立体特异性羟基产物，其本身是花生四烯酸的12-脂氧酶代谢产物。12(S)-HETE对多形核白细胞和血管平滑肌细胞有趋化作用和化学促活作用，它也作为血管紧张素-II诱导的醛固酮产生的第二信使。12(S)-HETE与氧化应激、炎症、癌症、动脉粥样硬化和心血管疾病等相关，有研究表明，12(S)-HETE参与抑制肾素的产生，刺激胰腺组织分泌胰岛素，诱导内皮细胞收缩和肿瘤细胞粘附。

Enzo Life Sciences的12S-羟基二十碳四烯酸比色法ELISA试剂盒，可在5小时内得到检测结果，在405nm处读取OD值。检测快速，准确性高，可靠性好。欣博盛生物作为Enzo Life Sciences授权中国一级代理，可提供该产品，欢迎咨询。

产品特点

◆ 高通量，仅需5小时即可获得多达39份样本（含复孔）的结果

◆ 高灵敏度，可测量低至146 pg/ml的12(S)-HETE

◆ 长期和多批次验证的可靠且可重复的实验结果

◆ 易于使用的颜色编码试剂和预包被板，减少了错误并节省了实验时间

产品信息

标准曲线示例

部分产品引用文献

1. Leukotriene B4 receptor-2 contributes to KRAS-driven lung tumor formation by promoting interleukin-6-mediated inflammation: J.H. Jang, et al.; Exp. Mol. Med. 53, 1559 (2022)

2. Development of heart failure with preserved ejection fraction in type 2 diabetic mice is ameliorated by preserving vascular function: M. Otto, et al.; Life Sci. 284, 119925 (2021)

3. 12(S)-HETE Mediates Diabetes Induced Endothelial Dysfunction by Activating Intracellular Endothelial Cell TRPV1: M. Otto, et al.; J. Clin. Invest. 130, 4999 (2020)

4. Arachidonate 12-Lipoxygenase and 12-Hydroxyeicosatetraenoic acid contribute to stromal aging-induced progression of pancreatic cancer: E.H. Sarsour, et al.; J. Biol. Chem. 295, 6946 (2020)

5. A Mediterranean Diet Rich in Extra-Virgin Olive Oil Is Associated with a Reduced Prevalence of Nonalcoholic Fatty Liver Disease in Older Individuals at High Cardiovascular Risk: X. Pintó, et al.; J. Nutr. 149, 1920 (2019)

6. Ablation of TRPV1 Elevates Nocturnal Blood Pressure in Western Diet-fed Mice: B. Zhong, et al.; Curr. Hypertens. Rev. 15, 144 (2019)

7. Mediatory roles of leukotriene B4 receptors in LPS-induced endotoxic shock: S.Y. Kwon, et al.; Sci. Rep. 9, 5936 (2019)

8. 5‐/12‐Lipoxygenase‐linked cascade contributes to the IL‐33‐induced synthesis of IL‐13 in mast cells, thus promoting asthma development: M. Ro, et al.; Allergy 73, 350 (2018)

9. Intravasation of SW620 colon cancer cell spheroids through the blood endothelial barrier is inhibited by clinical drugs and flavonoids in vitro: S. Holzner, et al.; Food Chem. Toxicol. 111, 114 (2018)

10. Mice lacking L-12/15-lipoxygenase show increased mortality during kindling despite demonstrating resistance to epileptogenesis: M.A. Kanzler, et al.; Epilepsia Open 3, 255 (2018)

11. Changes in platelet function with inflammation in patients undergoing vascular surgery: B. Olechowski, et al.; Platelets 30, 190 (2017)

12. 12(S)-HETE increases intracellular Ca2+ in lymph-endothelial cells disrupting their barrier function in vitro; stabilization by clinical drugs impairing calcium supply: C.H Nguyen, et al.; Cancer Lett. 380, 174 (2016)

13. 12-Lipoxygenase Inhibition on Microalbuminuria in Type-1 and Type-2 Diabetes Is Associated with Changes of Glomerular Angiotensin II Type 1 Receptor Related to Insulin Resistance: H.Z. Xu, et al.; Int. J. Mol. Sci. 17, E684 (2016)

14. Cancer cell-derived 12(S)-HETE signals via 12-HETE receptor, RHO, ROCK and MLC2 to induce lymph endothelial barrier breaching: C.H. Nguyen, et al.; Br. J. Cancer 115, 364 (2016)

15. 12/15-Lipoxygenase metabolites of arachidonic acid activate PPARγ: a possible neuroprotective effect in ischemic brain: L. Sun, et al.; J. Lipid. Res. 56, 502 (2015)

16. Lobatin b inhibits NPM/ALK and NF-κB attenuating anaplastic-large-cell-lymphomagenesis and lymphendothelial tumour intravasation: I. Kiss, et al.; Cancer Lett. 356, 994 (2015)

17. MyD88-BLT2-dependent cascade contributes to LPS-induced interleukin-6 production in mouse macrophage: A.J. Lee, et al.; Exp. Mol. Med. 47, e156 (2015)

18. Pharmacologic blockade of 12/15-lipoxygenase ameliorates memory deficits, Aβ and tau neuropathology in the triple-transgenic mice: J. Chu, et al.; Mol. Psychiatry 20, 1329 (2015)

19. Selective inhibition of 12-lipoxygenase protects islets and beta cells from inflammatory cytokine-mediated beta cell dysfunction: D.A. Taylor-Fishwick, et al.; Diabetologia 58, 549 (2015)

20. Transient postnatal fluoxetine decreases brain concentrations of 20-HETE and 15-epi-LXA4, arachidonic acid metabolites in adult mice: Z.X. Yuan, et al.; Prostaglandins Leukot. Essent. Fatty Acids 101, 9 (2015)

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