PTENP1 polyclonal antibody
产品名称： PTENP1 polyclonal antibody
英文名称： PTENP1 polyclonal antibody
产品编号： PAB9923
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against partial recombinant PTENP1.
- Immunogen:
- Recombinant protein corresponding to N-terminus of human PTENP1.
- Host:
- Rabbit
- Reactivity:
- Chimpanzee, Dog, Human, Macaque, Mouse, Opossum, Pufferfishes, Rat, Zebra fish
- Form:
- Liquid
- Storage Buffer:
- In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Recombinant Protein.
- Recommend Usage:
- ELISA (1:160000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Lack of PTEN expression in endometrial intraepithelial neoplasia is correlated with cancer progression.
Baak JP, Van Diermen B, Steinbakk A, Janssen E, Skaland I, Mutter GL, Fiane B, Lovslett K.Hum Pathol. 2005 May;36(5):555-61.
- 2.
- Epidermal growth factor and hypoxia-induced expression of CXC chemokine receptor 4 on non-small cell lung cancer cells is regulated by the phosphatidylinositol 3-kinase/PTEN/AKT/mammalian target of rapamycin signaling pathway and activation of hypoxia inducible factor-1alpha.
Phillips RJ, Mestas J, Gharaee-Kermani M, Burdick MD, Sica A, Belperio JA, Keane MP, Strieter RM.J Biol Chem. 2005 Jun 10;280(23):22473-81. Epub 2005 Mar 31.
- 3.
- Alterations of PTEN/MMAC1, a candidate tumor suppressor gene, and its homologue, PTH2, in small cell lung cancer cell lines.
Kim SK, Su LK, Oh Y, Kemp BL, Hong WK, Mao L.Oncogene. 1998 Jan 8;16(1):89-93.
- Applications
- Western Blot (Cell lysate)
- Western blot using PTENP1 polyclonal antibody (Cat # PAB9923) shows detection of endogenous PTENP1 in whole cell lysates from human derived cell lines HeLa (Lane 4), HEK293 (Lane 5) and MCF-7 (Lane 6).
The band at ~55 KDa (arrowhead) corresponds to PTENP1.
Lanes 1-3 were show the results of staining after the antibody was first pre-incubated with the immunizing peptide.
The identity of lower molecular weight bands in lane 4 is unknown.
Briefly, each lane contains approximately 35 ug of lysate.
Primary antibody was used at a 1 : 500 dilution in 5% BLOTTO in PBS reacted overnight at 4°C.
The membrane was washed and reacted with a 1 : 10,000 dilution of IRDye800™ conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature (800 nm channel, green).
Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red).
IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System d