NRF1 monoclonal antibody (M01), clone 2F9
产品名称： NRF1 monoclonal antibody (M01), clone 2F9
英文名称： NRF1 monoclonal antibody (M01), clone 2F9
产品编号： H00004899-M01
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Mouse monoclonal antibody raised against a partial recombinant NRF1.
- Immunogen:
- NRF1 (NP_005002, 201 a.a. ~ 285 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
- Sequence:
- TQAQLRAFIPEMLKYSTGRGKPGWGKESCKPIWWPEDIPWANVRSDVRTEEQKQRVSWTQALRTIVKNCYKQHGREDLLYAFEDQ
- Host:
- Mouse
- Reactivity:
- Human
- Isotype:
- IgG1 Kappa
- Storage Buffer:
- In 1x PBS, pH 7.2
- Storage Instruction:
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (35.09 KDa) .
- MSDS:
Download
- Publication Reference
- 1.
- Decreased expression of BRCA1 in SK-BR-3 cells is the result of aberrant activation of the GABP Beta promoter by an NRF-1-containing complex.
Thompson C, Macdonald G, Mueller CR.Mol Cancer. 2011 May 24;10(1):62. [Epub ahead of print]
- Applications
- Western Blot (Tissue lysate)
- NRF1 monoclonal antibody (M01), clone 2F9. Western Blot analysis of NRF1 expression in human Skeletal muscle.
- Protocol Download
- Western Blot (Cell lysate)
- NRF1 monoclonal antibody (M01), clone 2F9. Western Blot analysis of NRF1 expression in HeLa.
- Protocol Download
- Western Blot (Transfected lysate)
- Western Blot analysis of NRF1 expression in transfected 293T cell line by NRF1 monoclonal antibody (M01), clone 2F9.
Lane 1: NRF1 transfected lysate (Predicted MW: 55.7 KDa).
Lane 2: Non-transfected lysate. - Protocol Download
- Western Blot (Recombinant protein)
- Protocol Download
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
enlarge this image- Immunoperoxidase of monoclonal antibody to NRF1 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3 ug/ml]
- Protocol Download
- Sandwich ELISA (Recombinant protein)
- Detection limit for recombinant GST tagged NRF1 is approximately 0.03ng/ml as a capture antibody.
- Protocol Download
- RNAi Knockdown (Antibody validated)
- Western blot analysis of NRF1 over-expressed 293 cell line, cotransfected with NRF1 Validated Chimera RNAi ( Cat # H00004899-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NRF1 monoclonal antibody (M01), clone 2F9 (Cat # H00004899-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Protocol Download
- Application Image
- Western Blot (Tissue lysate)
- enlarge
- Western Blot (Cell lysate)
- enlarge
- Western Blot (Transfected lysate)
- enlarge
- Western Blot (Recombinant protein)
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- enlarge
- Sandwich ELISA (Recombinant protein)
- enlarge
- ELISA
- RNAi Knockdown (Antibody validated)
- enlarge
- Entrez GeneID:
- 4899
- GeneBank Accession#:
- NM_005011
- Protein Accession#:
- NP_005002
- Gene Name:
- NRF1
- Gene Alias:
- ALPHA-PAL
- Gene Description:
- nuclear respiratory factor 1
- Omim ID:
- 600879
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternate transcriptional splice variants, which encode the same protein, have been characterized. Additional variants encoding different protein isoforms have been described but they have not been fully characterized. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for "nuclear factor (erythroid-derived 2)-like 1" which has an official symbol of NFE2L1. [provided by RefSeq
- Other Designations:
- OTTHUMP00000184912,alpha palindromic-binding protein
- Gene Pathway