Benefits of the CatchPoint cyclic-AMP Assay Kit

·               High affinity reagents are optimized for sensitivity when cAMP levels are low, ensuring results when other assays fail.

·               Robust format resists interference from colored or fluorescent compounds, improving reliability of results.

·               Rapid signal development (< 10 min) and no termination step give users a flexible read time that is compatible with automation and high-throughput screening.

·               Quantitative method allows accurate determination of cAMP in samples.

CatchPoint cAMP Assay principle.  The cAMP in the sample or standard competes with horseradish peroxidase (HRP)-labeled cAMP conjugate for binding sites on the anti-cAMP antibodies. In the absence of cAMP, most of the HRP-cAMP conjugate is bound to the antibody. Increasing concentrations of cAMP competitively decrease the amount of bound conjugate, thus decreasing measured HRP activity.

CatchPoint cyclic-AMP Assay Kit is available in 96- and 384-well formats.  They are also available in CatchPoint cAMP Explorer or Bulk quantities, to meet the throughput needs of different users.

CatchPoint cAMP Explorer Fluorescent Assay Kit

·               One CatchPoint cAMP Explorer Kit provides the following components:

·               Two black clear-bottom plates (96- or 384-well format depending upon specific product)

·               100 mL ELISA Assay Buffer

·               1 (96-well) or 2 (384-well) vials Rabbit Anti-cAMP Antibody

·               1 (96-well) or 2 (384-well) vials cAMP Calibrator

·               1 (96-well) or 2 (384-well) vials HRP-cAMP Conjugate

·               100mL, 10X Wash Solution Concentrate

·               50 mL (96-well) or 25 mL (384-well) Cell Lysis Buffer

·               1 vial 100X Substrate

·               100 mL Substrate Buffer

CatchPoint cAMP Bulk Fluorescent Assay Kit

·               One CatchPoint cAMP Bulk Kit provides:

·               Ten (96-well) or twenty (384-well) black clear-bottom plates

·               950 mL (96-well) or 2 x 950 mL (384-well) ELISA Assay Buffer

·               2 vials Rabbit Anti-cAMP Antibody

·               2 vials cAMP Calibrator

·               2 vials HRP-cAMP Conjugate

·               950mL, 10X Wash Solution Concentrate

·               450 mL (96-well) or 2 x 450 mL Cell Lysis Buffer

·               2 x 900 µL (96-well) or 5 x 900 mL (384-well) 100X Substrate

·               950 mL Substrate Buffer