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IL27 / Interleukin-27 Protein
 
 
 
IL27 / Interleukin-27 Protein Price Inquiry ( Available Sizes )
 
 
 
50μg:  询价;
 
200μg:  询价;
 
≥1mg 大包装:  询价
 
 
 
IL27 / Interleukin-27蛋白产品信息
 
 
 
 
别名:
 
 
p28,  IL30,  IL-27,  IL-27A,  IL27p28,  MGC71873,  IL27
 
 
 
 
表达方式:
 
 
A DNA sequence encoding the IL27 complex composed of IL27B subunit  (NP_005746.2) ( Met 1 - Lys 229 ) and the N-terminally truncated IL27p28 (IL27A) subunit ( NP_663634.2 ) ( Phe 29 - Pro 243 ) linked by an polypeptide linker was fused with the Fc region of human IgG1 at the C-terminus.
 
 
 
 
种属:
 
Human
 
 
 
表达宿主: 
 
Human Cells
 
 
 
 
IL27 / Interleukin-27蛋白质控指标
 
 
 
 
纯度:
 
> 92 % as determined by SDS-PAGE
 
SDS-PAGE:
 
 
IL27 protein
 
 
 
 
内毒素:
 
< 1.0 EU per μg of the protein as determined by the LAL method
 
 
 
稳定性:
 
Samples are stable for up to twelve months from date of receipt at -70℃
 
 
 
预测N端:
 
Arg 21
 
 
 
分子量:
 
 
The recombinant human IL27 complex ( IL27B & IL27A ) consists of 450 amino acids after removal of the signal peptide and has a calculated molecular mass of 50.4 kDa as estimated in SDS-PAGE under reducing conditions.
 
 
 
 
缓冲液:
 
 
Lyophilized from sterile PBS, 10% glycerol
 
 
 
 
 
IL27 / Interleukin-27蛋白使用指南
 
 
 
 
储存方法:
 
Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
 
 
 
 
 
Reconstitution:
 
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
 
 
 
 
IL27 / Interleukin-27蛋白相关产品和研究主题
 
 
相关研究领域:
 
Immunology>>Adaptive Immunity>>T Cell>>Helper T Cell>>IL-27
 
Immunology>>Cytokine & Receptor>>Interleukin & Receptor>>IL-12 Family & Receptor>>IL-27
 
蛋白:
 
抗体:
 
 
IL27 / Interleukin-27蛋白背景综述
 
IL27A, also referred as the IL12 p35-related protein, p28, is one subunit of a heterodimeric cytokine complex, and associates with another subunit EBI3 (EBV-induced gene 3), an IL12 p40-related protein (IL27B). IL-27 is a cytokine with pro- and anti-inflammatory properties, that can regulate T helper cell development, suppress T-cell proliferation, stimulate cytotoxic T cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon-gamma / IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1 / TCCR which appears to be required but not sufficient for IL-27-mediated signal transduction. IL-27 potentiate the early phase of TH1 response and suppress TH2 and TH17 differentiation. It induces the differentiation of TH1 cells via two distinct pathways, p38 MAPK/TBX21- and ICAM1 / ITGAL / ERK-dependent pathways. IL-27 also induces STAT1, STAT3, STAT4 and STAT5 phosphorylation and activates TBX21/T-Bet via STAT1 with resulting IL12RB2 up-regulation, an event crucial to TH1 cell commitment. IL-27 suppresses the expression of GATA3, the inhibitor TH1 cells development. IL-27 reveals to be a potent inhibitor of TH17 cell development and of IL-17 production. IL-27 also antagonizes the effects of some cytokines such as IL6 through direct effects on T cells. Another important role of IL-27 is its antitumor activity and antiangiogenic activity.
 
参考文献
 
 
 
Pflanz S., et al., 2002, Immunity 16:779-790.
 
Hibbert L., et al., 2003, J. Interferon Cytokine Res. 23:513-522.
 
Fakruddin J.M., et al., 2007, Blood 109:1841-1849.
 
Feng X.M., et al., 2007, Hum. Immunol. 68:965-972.
 
Chae S.-C., et al., 2007, J. Hum. Genet. 52:355-361.
 
 
 
 
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