LECT1 polyclonal antibody
产品名称： LECT1 polyclonal antibody
英文名称： LECT1 polyclonal antibody
产品编号： PAB2573
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of LECT1.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human LECT1.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Ammonium sulfate precipitation
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:50-100)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Cell-specific epigenetic regulation of ChM-I gene expression: crosstalk between DNA methylation and histone acetylation.
Aoyama T, Okamoto T, Kohno Y, Fukiage K, Otsuka S, Furu M, Ito K, Jin Y, Nagayama S, Nakayama T, Nakamura T, Toguchida J.Biochem Biophys Res Commun. 2008 Jan 4;365(1):124-30. Epub 2007 Oct 31.
- 2.
- Chondromodulin-I maintains cardiac valvular function by preventing angiogenesis.
Yoshioka M, Yuasa S, Matsumura K, Kimura K, Shiomi T, Kimura N, Shukunami C, Okada Y, Mukai M, Shin H, Yozu R, Sata M, Ogawa S, Hiraki Y, Fukuda K.Nat Med. 2006 Oct;12(10):1151-9. Epub 2006 Sep 17.
- 3.
- Methylation in the core-promoter region of the chondromodulin-I gene determines the cell-specific expression by regulating the binding of transcriptional activator Sp3.
Aoyama T, Okamoto T, Nagayama S, Nishijo K, Ishibe T, Yasura K, Nakayama T, Nakamura T, Toguchida J.J Biol Chem. 2004 Jul 2;279(27):28789-97. Epub 2004 Apr 23.
- Applications
- Western Blot (Cell lysate)
- Western blot analysis of LECT1 polyclonal antibody (Cat # PAB2573) in NCI-H460 cell line lysates (35 ug/lane).LECT1 (arrow) was detected using the purified polyclonal antibody (1 : 60 dilution).
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human lung carcinomareacted with LECT1 polyclonal antibody (Cat # PAB2573), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Entrez GeneID:
- 11061
- Protein Accession#:
- NP_008946;O75829
- Gene Name:
- LECT1
- Gene Alias:
- BRICD3,CHM-I,CHM1
- Gene Description:
- leukocyte cell derived chemotaxin 1
- Omim ID:
- 605147
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene encodes a glycosylated transmembrane protein that is cleaved to form a mature, secreted protein. The N-terminus of the precursor protein shares characteristics with other surfactant proteins and is sometimes called chondrosurfactant protein although no biological activity has yet been defined for it. The C-terminus of the precursor protein contains a 25 kDa mature protein called leukocyte cell-derived chemotaxin-1 or chondromodulin-1. The mature protein promotes chondrocyte growth and inhibits angiogenesis. This gene is expressed in the avascular zone of prehypertrophic cartilage and its expression decreases during chondrocyte hypertrophy and vascular invasion. The mature protein likely plays a role in endochondral bone development by permitting cartilaginous anlagen to be vascularized and replaced by bone. It may be involved also in the broad control of tissue vascularization during development. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq
- Other Designations:
- BRICHOS domain containing 3,OTTHUMP00000018466,chondromodulin I
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