Product: Anti-EDG-6 (CT), Polyclonal
Cat. No.: PC-087 (100 μg)
Background:
EDG-6 belongs to a family of G-protein coupled
receptors whose ligands are lysophospholipids.
The ligand for EDG-6 is sphingosine-1-
phosphate. There are 8 known members of the
EDG receptor family and they are implicated in
mediating growth related effects such as
induction of cellular proliferation, alterations in
differentiation and survival and suppression of
apoptosis. They also evoke cellular effector
functions that are dependent on cytoskeletal
responses such as contraction, secretion,
adhesion and chemotaxis. EDG receptors are
developmentally regulated and differ in tissue
distribution. They couple to multiple types of G
proteins to signal through ras and MAP kinase,
rho, phospholipase C and several protein
tyrosine kinases. EDG-6 is expressed in
lymphoid and hematopoietic tissue and in the
lungs. Recently, the designation of the EDG
receptors has been modified to include the
ligand for the receptor. Thus, the new
designation for the EDG-6 receptor is the
sphingosine 1-phosphate receptor-4 (S1P-4).
Specificity:
Reacts with EDG-6.
Species Reactivity:
Human, other species not tested.
Host:
Rabbit
Immunogen:
Synthetic peptide derived from the C-terminal of
the EDG-6 receptor.
Format:
100 μg purified antibody at 1 mg/ml in PBS with
0.08% sodium azide. Solution was sterile
filtered at 0.2 μm.
Storage:
Store at -20°C. Avoid freeze/thaw cycles.
Applications and Suggested Dilutions:

Western Blot: Use at 5-10 μg/ml using a
human EDG-6 receptor transfected cell line.
Detects a band of approximately 45 kDa in
Cos cells transfected with full length human
EDG-6. Due to low expression of EDG
receptors, we recommend using Pierce
Femto Signal substrate for western blot
development.
Positive Control: RH7777 cells transfected
with EDG-6 protein.
The optimal dilution for a specific application
should be determined by the researcher.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product.