HK1 polyclonal antibody
产品名称： HK1 polyclonal antibody
英文名称： HK1 polyclonal antibody
产品编号： PAB4008
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of HK1.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human HK1.
- Host:
- Rabbit
- Reactivity:
- Human, Mouse
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- HK Utrecht: missense mutation in the active site of human hexokinase associated with hexokinase deficiency and severe nonspherocytic hemolytic anemia.
van Wijk R, Rijksen G, Huizinga EG, Nieuwenhuis HK, van Solinge WW.Blood. 2003 Jan 1;101(1):345-7. Epub 2002 Aug 8.
- 2.
- Gene expression and biological significance of hexokinase in erythroid cells.
Murakami K, Kanno H, Tancabelic J, Fujii H.Acta Haematol. 2002;108(4):204-9.
- 3.
- Human HKR isozyme: organization of the hexokinase I gene, the erythroid-specific promoter, and transcription initiation site.
Murakami K, Kanno H, Miwa S, Piomelli S.Mol Genet Metab. 1999 Jun;67(2):118-30.
- Applications
- Western Blot (Tissue lysate)
- The HK1 polyclonal antibody (Cat # PAB4008) is used in Western blot to detect HK1 in mouse skeletal muscle tissue lysate.
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with HK1 polyclonal antibody (Cat # PAB4008) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Entrez GeneID:
- 3098
- Protein Accession#:
- P19367
- Gene Name:
- HK1
- Gene Alias:
- HK1-ta,HK1-tb,HK1-tc,HKI,HXK1
- Gene Description:
- hexokinase 1
- Omim ID:
- 142600
- Gene Ontology:
- Hyperlink
- Gene Summary:
- Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus; the remainder of the protein is identical among all the isoforms. A sixth transcript variant has been described, but due to the presence of several stop codons, it is not thought to encode a protein. [provided by RefSeq
- Other Designations:
- OTTHUMP00000019725,brain form hexokinase,glycolytic enzyme
- Gene Pathway
- Amino sugar and nucleotide sugar metabolism
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Fructose and mannose metabolism
- Galactose metabolism
- Glycolysis / Gluconeogenesis
- Insulin signaling pathway
- Metabolic pathways
- Starch and sucrose metabolism
- Streptomycin biosynthesis
- Type II diabetes mellitus