MAP3K8 polyclonal antibody
产品名称： MAP3K8 polyclonal antibody
英文名称： MAP3K8 polyclonal antibody
产品编号： PAB2282
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of MAP3K8.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human MAP3K8.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- COT kinase proto-oncogene expression in T cells: implication of the JNK/SAPK signal transduction pathway in COT promoter activation.
Sanchez-Gongora E, Lisbona C, de Gregorio R, Ballester A, Calvo V, Perez-Jurado L, Alemany S.J Biol Chem. 2000 Oct 6;275(40):31379-86.
- 2.
- The human cot proto-oncogene encodes two protein serine/threonine kinases with different transforming activities by alternative initiation of translation.
Aoki M, Hamada F, Sugimoto T, Sumida S, Akiyama T, Toyoshima K.J Biol Chem. 1993 Oct 25;268(30):22723-32.
- 3.
- Expression cDNA cloning of a serine kinase transforming gene.
Chan AM, Chedid M, McGovern ES, Popescu NC, Miki T, Aaronson SA.Oncogene. 1993 May;8(5):1329-33.
- Applications
- Western Blot (Cell lysate)
- Western blot analysis of MAP3K8 polyclonal antibody (Cat # PAB2282) in A-375 cell lysate. MAP3K8 (Arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with the MAP3K8 polyclonal antibody (Cat # PAB2282) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
- Entrez GeneID:
- 1326
- Protein Accession#:
- P41279
- Gene Name:
- MAP3K8
- Gene Alias:
- COT,EST,ESTF,FLJ10486,TPL2,Tpl-2,c-COT
- Gene Description:
- mitogen-activated protein kinase kinase kinase 8
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene was identified by its oncogenic transforming activity in cells. The encoded protein is a member of the serine/threonine protein kinase family. This kinase can activate both the MAP kinase and JNK kinase pathways. This kinase was shown to activate IkappaB kinases, and thus induce the nuclear production of NF-kappaB. This kinase was also found to promote the production of TNF-alpha and IL-2 during T lymphocyte activation. Studies of a similar gene in rat suggested the direct involvement of this kinase in the proteolysis of NF-kappaB1,p105 (NFKB1). This gene may also utilize a downstream in-frame translation start codon, and thus produce an isoform containing a shorter N-terminus. The shorter isoform has been shown to display weaker transforming activity. [provided by RefSeq
- Other Designations:
- Cancer Osaka thyroid oncogene,Ewing sarcoma transformant,OTTHUMP00000019392,OTTHUMP00000019393,cot (cancer Osaka thyroid) oncogene,proto-oncogene serine/threoine protein kinase,tumor progression locus-2
- Related Disease